Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Identify the enzyme used for the isolation of genetic material from fungal cells.

Options:

Lysozyme

Pepsin

Cellulase

Chitinase

Correct Answer:

Chitinase

Explanation:

The correct answer is Option (4) - Chitinase

Lysozyme: Lysozyme is an enzyme that breaks down the peptidoglycan layer of bacterial cell walls However, it is not typically used for isolating genetic material from fungal cells because fungi have a different cell wall composition compared to bacteria. Fungal cell walls contain chitin, not peptidoglycan.

Pepsin: Pepsin is a digestive enzyme found in the stomach that breaks down proteins. It is not typically used for isolating genetic material from fungal cells because it does not specifically target the fungal cell wall components.

Cellulase: Cellulase is an enzyme that breaks down cellulose, which is found in the cell walls of plants and some algae. It is not typically used for isolating genetic material from fungal cells because fungi have chitin, not cellulose, in their cell walls.

Chitinase: Chitinase is an enzyme that specifically targets chitin, which is a structural polysaccharide found in the cell walls of fungi, the exoskeletons of arthropods, and some other organisms. In the context of isolating genetic material from fungal cells, chitinase is used to degrade the fungal cell wall, allowing access to the genetic material contained within the cells. Therefore, chitinase is the enzyme of choice for this purpose.