Arrange the steps of biopsy and histopathology of tissue involved in detection of cancer.

(A) A thin section of tissue is stained
(B) Freezing of tissue in liquid nitrogen
(C) A piece of the suspected tissue cut into thin sections
(D) Examination under microscope

Choose the correct answer from the options given below:

(B), (C), (A), (D)

The correct answer is Option (2) → (B), (C), (A), (D)   

(B) Freezing of tissue in liquid nitrogen

(C) A piece of the suspected tissue cut into thin sections

(A) A thin section of tissue is stained

(D) Examination under microscope

Early detection of cancers is essential as it allows the disease to be treated successfully in many cases. Cancer detection is based on biopsy and histopathological studies of the tissue and blood and bone marrow tests for increased cell counts in the case of leukemias. In biopsy, a piece of the suspected tissue cut into thin sections is stained and examined under microscope (histopathological studies) by a pathologist. Techniques like radiography (use of X-rays), CT (computed tomography) and MRI (magnetic resonance imaging) are very useful to detect cancers of the internal organs. Computed tomography uses X-rays to generate a three-dimensional image of the internals of an object. MRI uses strong magnetic fields and non-ionising radiations to accurately detect pathological and physiological changes in the living tissue. Antibodies against cancer -specific antigens are also used for detection of certain cancers.