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Home Questions 4FNYSQ86SS
Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Which of the following is required to perform polymerase chain reaction?

Options:

Primers, dNTPs and DNA polymerase.

DNA,CaCl2(calcium chloride) and nuclease.

Mg 2+ (magnesium ion) , DNA.

Both I and III.

Correct Answer:

Both I and III.

Explanation:

a-Primers, dNTPs and DNA polymerase.

b-DNA,CaCl2(calcium chloride) and nuclease.

c-Mg 2+ (magnesium ion) , DNA.

d-Both I and III is the correct option.

According to the components and relevant steps of Polymerase chain reaction, primers, dNTPs and DNA polymerase are used for DNA synthesis. DNA is used as a template and Mg+ is necessary for DNA synthesis.

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