Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Read the passage and answer the following question :

After cutting DNA with restriction endonucleases, the resulting fragments can be separated using a technique called gel electrophoresis. This process takes advantage of the negative charge of DNA molecules, causing them to move towards the anode in an electric field through a matrix, commonly made of agarose, a natural polymer derived from seaweeds. The agarose gel acts as a sieve, allowing smaller DNA fragments to move farther than larger ones. To visualize the separated DNA fragments, the gel is stained with ethidium bromide and exposed to UV radiation, resulting in bright orange-colored bands. These bands represent the different sizes of DNA fragments. The specific bands of interest are carefully cut out from the gel, and the DNA is extracted from the gel piece. This step is called elution. The purified DNA fragments obtained through elution are then used to construct recombinant DNA by joining them with cloning vectors. This process enables scientists to study, manipulate, and replicate specific DNA sequences for various applications in genetic engineering and molecular biology.

 

Agarose is extracted from :

Options:

Bacteria

Marine animals

Sea weeds

Fungi

Correct Answer:

Sea weeds

Explanation:

The correct answer is Option (3) - Sea weeds

The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. Nowadays the most commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel.