Match List - I with List - II.
| List - I | List - II |
|---|---|
| (A) First Restriction Endonuclease | (I) GAATTC |
| (B) Disarmed Plasmid | (II) Selectable markers |
| (C) Antibiotic resistant genes | (III) Hind II |
| (D) EcoRI | (IV) Vector |
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 3
The correct answer is Option (3) → (A)-(III), (B)-(IV), (C)-(II), (D)-(I)
(A) First Restriction Endonuclease — this matches (III) Hind II. It was the first enzyme found to cut DNA at a specific sequence.
(B) Disarmed Plasmid — this matches (IV) Vector. The Ti plasmid of Agrobacterium is disarmed (made harmless) and used as a vector for plants.
(C) Antibiotic resistant genes — this matches (II) Selectable markers. They let only transformed cells grow on the antibiotic.
(D) EcoRI — this matches (I) GAATTC. EcoRI recognises the palindrome GAATTC and cuts between G and A.