Match List I with List - II.

Choose the correct answer from the options given below:

(A)-(III), (B)-(IV), (C)-(II), (D)-(I)

The correct answer is Option (3) -(A)-(III), (B)-(IV), (C)-(II), (D)-(I)

Explanation:

 (A) The first restriction endonuclease–Hind II, whose functioning depended on a specific DNA nucleotide sequence was isolated and characterised five years later. It was found that Hind II always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for Hind II.
 (B) A disarmed plasmid is used as a vector. So disarmed pathogen’ vectors, which when allowed to infect the cell, transfer the recombinant DNA into the host

(C) Antibiotic-resistant genes are often used as selectable markers in plasmids, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli.

(D) EcoRI is a specific restriction endonuclease that recognizes and cleaves DNA at the sequence GAATTC.