Match List-I with List-II :

Choose the correct answer from the options given below:

(A)-(III), (B)-(IV), (C)-(II), (D)-(I)

The correct answer is Option (1) →(A)-(III), (B)-(IV), (C)-(II), (D)-(I)

In genetic engineering, a selectable marker is a gene that confers a specific trait, such as resistance to an antibiotic, which allows researchers to identify and select cells that have successfully incorporated recombinant DNA. A selectable marker in genetic engineering serves as a means to identify and distinguish cells that have successfully taken up the foreign DNA (transformants) from those that haven't (non-transformants). It also allows researchers to selectively promote the growth of the transformed cells while inhibiting the growth of non-transformed cells.

Origin of replication (ori) is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. This sequence is also responsible for controlling the copy number of the linked DNA. So, if one wants to recover many copies of the target DNA it should be cloned in a vector whose origin support high copy number.

Restriction enzymes belong to a larger class of enzymes called nucleases. These are of two kinds; exonucleases and endonucleases. Exonucleases remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA.

In Recombinant DNA Technology (RDT), the recognition site refers to the specific sequence of bases in DNA where a restriction endonuclease (restriction enzyme) cuts.