Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Which of the following statements are correct about restriction enzymes?

(A). Each restriction endonuclease recognizes a specific palindromic nucleotide sequence in the DNA.
(B). Endonuclease removes nucleotides from the terminal end of the DNA.
(C). The first discovered restriction endonuclease is Hind II.
(D). Restriction enzymes belong to a larger class of enzyme polymerase.

Choose the correct answer from the options given below.

Options:

(B) and (D) only

(A), and (C) only

(B), (C) and (D)

(A), (C) and (D) only

Correct Answer:

(A), and (C) only

Explanation:

The correct answer is Option (2) → (A), and (C) only 

Restriction Endonucleases (Molecular Scissors): Restriction enzymes belong to a larger class of enzymes called nucleases. These are enzymes that are commonly referred to as "molecular scissors" because they cut DNA molecules at specific recognition sequences. These recognition sequences are often palindromic, meaning the sequence reads the same forwards and backward.

Restriction enzymes belong to a larger class of enzymes called nucleases. These are of two kinds; exonucleases and endonucleases. Exonucleases remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA.

The first restriction endonuclease–Hind II, whose functioning depended on a specific DNA nucleotide sequence was isolated and characterized five years later. It was found that Hind II always cut DNA molecules at a particular point by recognizing a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for Hind II.