Which of the following enzymes join the sticky ends in recombinant DNA technology? |
Exonuclease Endonuclease Ligase Chitinase |
Ligase |
The correct answer is Option (3) → Ligase Ligase → Joins (seals) two fragments of DNA together by forming a phosphodiester bond. That’s why it is called the “molecular glue” in genetic engineering. For example, DNA ligase is used in recombinant DNA technology to join foreign DNA with a plasmid. Exonuclease → Cuts nucleotides one by one from the end of DNA/RNA. Endonuclease → Cuts DNA/RNA at specific internal sites. Chitinase → Chitinase is an enzyme that breaks down chitin, a structural polysaccharide found in the cell walls of fungi and some bacteria.
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