Hugo deVries's work on mechanism of evolution states that evolution is

caused due to a single step large mutation

The correct answer is Option (3) → caused due to a single step large mutation

In the first decade of twentieth century, Hugo deVries based on his work on evening primrose brought forth the idea of mutations – large difference arising suddenly in a population. He believed that it is mutation which causes evolution and not the minor variations (heritable) that Darwin talked about. Mutations are random and directionless while Darwinian variations are small and directional. Evolution for Darwin was gradual while deVries believed mutation caused speciation and hence called it saltation (single step large mutation).