The method of DNA transfer using high-velocity micro particles is known as:

Biolistics

The correct answer is Option (1) -Biolistics

There are several methods to introduce alien DNA into host cells. One such method is micro-injection, where recombinant DNA is directly injected into the nucleus of an animal cell.

In another technique, suitable for plants, cells are bombarded with high-velocity micro-particles of gold or tungsten coated with DNA. This method is known as biolistics or gene gun.

(a) Biolistics: Biolistics is a method of introducing foreign DNA into host cells, particularly plant cells, using high-velocity micro particles. In this technique, small particles, such as gold or tungsten, are coated with the desired DNA and then shot into the target cells using a device known as a gene gun. The high velocity of the particles allows them to penetrate the cell wall and deliver the DNA into the host cell's nucleus.

(b) Hybridisation: Hybridisation is a process of combining two different organisms or species to create a hybrid. In genetics, it refers to the process of combining two different DNA strands to form a double-stranded molecule. This can occur naturally, such as during sexual reproduction, or it can be artificially induced in a laboratory setting.

(c) Tissue Culture: Tissue culture is a technique in which small tissue samples or cells are taken from a plant or animal and grown in a controlled environment outside of the organism's body. This method allows researchers to study the behavior of cells and tissues in a controlled setting and can be used for various purposes, including genetic engineering.

(d) Vegetative Propagation: Vegetative propagation is a form of asexual reproduction in plants where new individuals are produced from vegetative parts of the parent plant, such as stems, roots, or leaves. This method allows plants to reproduce without the need for seeds and is commonly used in horticulture for cloning desirable plants.