When EcoRI acts on the gene of interest (GOI) and the vector, what type of ends does it produce in each?

Sticky ends in both

EcoRI is a type II restriction enzyme that recognizes a specific palindromic DNA sequence and cleaves the DNA at that site. The palindromic sequence recognized by EcoRI is 5'-GAATTC-3', and it cuts between the G and A, producing sticky ends.

When EcoRI acts on the gene of interest (GOI) and the vector, it will produce sticky ends in both. The sticky ends generated by EcoRI in the GOI and vector can be complementary to each other, allowing them to anneal and form hydrogen bonds. This property of sticky ends is essential for the process of DNA ligation, where the GOI and vector can be joined together using DNA ligase to create a recombinant DNA molecule.