In a process to obtain recombinant DNA, a sequence is cut with Hind II and other with EcoR I. What will be the next step?

The resultant DNA fragments have the same kind of ‘sticky-ends’ and, these can be joined together.

Hind II and EcoR I are both type II restriction enzymes that recognize specific DNA sequences and make staggered cuts, leaving short, single-stranded overhangs, also known as "sticky-ends." These sticky-ends are complementary to each other, which allows the DNA fragments to be easily joined together by DNA ligase.

Once the DNA fragments with compatible sticky-ends have been annealed, they form a recombinant DNA molecule. This recombinant DNA can then be inserted into a cloning vector, which is a DNA molecule that can replicate in a host cell. The recombinant DNA molecule, now combined with the cloning vector, is ready to be introduced into a host cell, where it can be expressed to produce the desired protein or RNA.

In summary, Hind II and EcoR I cut the DNA to generate fragments with compatible sticky-ends, which are then joined together to form the recombinant DNA. This recombinant DNA can be inserted into a cloning vector and then introduced into a host cell to produce the desired product.