Practicing Success
What is the correct sequence of structural genes in lac operon ? |
lac A , lac Y, lac Z . lac Y , lac A, lac Z . lac Z , lac Y, lac A . lac A , lac Z, lac Y . |
lac Z , lac Y, lac A . |
The correct answer is Option (3)- lac Z , lac Y, lac A. In lac operon (here lac refers to lactose), a polycistronic structural gene is regulated by a common promoter and regulatory genes. The lac operon consists of one regulatory gene (the i gene – here the term i does not refer to inducer, rather it is derived from the word inhibitor) and three structural genes in the sequence of z, y, and a.The i gene codes for the repressor of the lac operon. The z gene codes for beta-galactosidase ( β-gal), which is primarily responsible for the hydrolysis of the disaccharide, lactose into its monomeric units, galactose and glucose. The y gene codes for permease, which increases permeability of the cell to β-galactosides. The a gene encodes a transacetylase. Hence, all the three gene products in lac operon are required for metabolism of lactose. |