Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Read the statements about gene gun method.

I. The gene gun method is also referred to as the biolistic technique.

II. In this method, cells are bombarded with high-velocity microparticles coated with DNA in plants, with gold or tungsten being commonly used.

III. Suitable for plants

Which of the statements given above are correct?

Options:

I and II

I and III

II and III

I, II and III

Correct Answer:

I, II and III

Explanation:

The correct answer is Option (4) – I, II and III

 In a method known as micro-injection, recombinant DNA is directly injected into the nucleus of an animal cell.

In another method, suitable for plants, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun.

And the last method uses ‘disarmed pathogen’ vectors, which when allowed to infect the cell, transfer the recombinant DNA into the host.