Match List-I with List-II
| List-I | List-II |
|---|---|
| Terms | Functions |
| (A) Cloning | (I) Cuts at the specific position within DNA |
| (B) Plasmid | (II) Making multiple identical copies of template DNA |
| (C) Origin of replication | (III) Autonomously replicating circular extrachromosomal DNA |
| (D) Endonuclease | (IV) Initiates replication |
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → (A) - (II), (B) - (III), (C) - (IV), (D) - (I)
(A) Cloning — this matches (II) Making multiple identical copies of template DNA. When a piece of DNA is joined to a vector and put into a host, it is copied every time the vector is copied, so many identical copies are made.
(B) Plasmid — this matches (III) Autonomously replicating circular extrachromosomal DNA. A plasmid is a small ring of DNA in bacteria, outside the main chromosome. It copies itself on its own, so it serves as a vector to carry foreign DNA.
(C) Origin of replication — this matches (IV) Initiates replication. Ori is the sequence where replication starts. A piece of DNA linked to it can be copied in the host, and the ori also controls how many copies are made.
(D) Endonuclease — this matches (I) Cuts at the specific position within DNA. Restriction endonucleases cut DNA inside the strand at a particular recognition sequence, while exonucleases remove nucleotides from the ends.