Read the passage carefully and answer the given questions.

Recombinant DNA Technology

Asexual reproduction preserves the genetic information, while sexual reproduction permits variation. Traditional hybridization procedures used in plants and animals breeding very often lead to inclusion and multiplication of undesirable genes along with the desired genes. The techniques of genetic engineering, which include creation of recombinant DNA, use of gene cloning and gene transfer, overcome this limitation and allow us to isolate and introduce only one or a set of desirable genes without introducing genes into the target organism.

What is the purpose of using a gene promoter in recombinant DNA technology?

To ensure the foreign gene expression in the host organism

The correct answer is Option (3) → To ensure the foreign gene expression in the host organism 

In recombinant DNA technology, a gene promoter is a DNA sequence placed upstream of a gene to:

• Act as a binding site for RNA polymerase and transcription factors
• Initiate transcription of the gene
• Control when, where, and how much the gene is expressed

Without a suitable promoter, even if the gene is inserted into the host organism, it may not be expressed, meaning no protein will be produced.

Other options are incorrect:

• To cut DNA at specific sequences — This is the role of restriction enzymes, not promoters.
• To insert the gene into the host organism's genome — This is done by vectors or gene delivery methods, not promoters.
• To replicate the gene in a bacterial cell — This requires an origin of replication, not a promoter.