Which of the following enzyme is used during isolation of DNA from fungal cells?

Chitinase

The correct answer is Option (4) → Chitinase

Fungi - Chitinase:  Fungi have cell walls composed primarily of chitin, and chitinase is an enzyme that breaks down chitin.

Plant cells - Cellulase: Plant cell walls are made largely of cellulose, and cellulase is an enzyme that breaks down cellulose.

Bacteria - Lysozyme: Bacterial cell walls contain peptidoglycan, and lysozyme is an enzyme that degrades peptidoglycan, thus breaking down bacterial cell walls.

DNA ligase (joining or sealing enzymes) is an enzyme, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.  Thus, they help in sealing gaps in DNA fragments. Therefore, they act as a molecular glue.