Practicing Success
Which among the following is not a feature of DNA fingerprinting ? |
Digestion of DNA by ligases . Separation of DNA fragments by electrophoresis. Hybridisation using labelled VNTR probe . Detection of hybridised DNA fragments by autoradiography. |
Digestion of DNA by ligases . |
The correct answer is Option (1) -Digestion of DNA by ligases . The technique of DNA Fingerprinting was initially developed by Alec Jeffreys. He used a satellite DNA as probe that shows very high degree of polymorphism. It was called as Variable Number of Tandem Repeats (VNTR). The technique, as used earlier, involved Southern blot hybridisation using radiolabelled VNTR as a probe. It included : |