Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Assertion: A bacterial cell with no restriction enzymes will be easily infected and lysed by bacteriophages.
Reason: Restriciton enymes catalyse synthesis of protective coat around bacterial cell that prevents bacteriophage attack.

Options:

Both Assertion (A) and Reason (R) are correct and R is the correct explanation of A.

Both Assertion (A) and Reason (R) are correct  but R is not the correct explanation of A.

Assertion (A) is true but Reason (R) is not correct.

Both Assertion (A) and Reason are false (F ).

Correct Answer:

Both Assertion (A) and Reason are false (F ).

Explanation:

Restriction enzymes were named due to the phenomenon of host restriction of bacterial phages. Restriction enzymes produced in a bacterial cell, recognize and cleave foreign DNA introduced (such as from bacteriophage) into the cell. Thus, bacterial cell cannot be infected and lysed by bacteriophage and hence a bacterial cell lacking restriction enzymes is easily susceptible to infection of phages. The DNA of the host bacterial cell is protected from its own restriction endonucleases by methylation (usually of A and C) within their recognition sites.