Consider the following statements regarding osmotic pressure:

(A) Molar mass of a protein can be determined using osmotic pressure method.

(B) The osmotic pressure is proportional to the molarity.

(C) Reverse osmosis occurs when a pressure larger than osmotic pressure is applied to the concentrated solution side.

(D) Edema occurs due to retention of water in tissue cells as a result of osmosis.

Choose the correct statements with reference to osmotic pressure:

A, B, C and D

The correct answer is option 3. A, B, C and D.

Let us evaluate each statement regarding osmotic pressure:

A. Molar mass of a protein can be determined using osmotic pressure method.

This statement is true. The osmotic pressure of a solution is directly proportional to the molarity of the solute and the absolute temperature and inversely proportional to the molar mass of the solute. By measuring the osmotic pressure of a protein solution and knowing the other variables, the molar mass of the protein can be determined.

B. The osmotic pressure is proportional to the molarity.

This statement is true. Osmotic pressure (\(\pi\)) is given by the equation \(\pi = MRT\), where \(M\) is the molarity of the solute, \(R\) is the ideal gas constant, and \(T\) is the temperature in Kelvin. This equation shows that osmotic pressure is directly proportional to the molarity.

C. Reverse osmosis occurs when a pressure larger than osmotic pressure is applied to the concentrated solution side.

This statement is true. Reverse osmosis is a process where a solvent moves from a region of higher solute concentration to a region of lower solute concentration when a pressure greater than the osmotic pressure is applied to the concentrated solution side. This process is commonly used in water purification systems.

D. Edema occurs due to retention of water in tissue cells as a result of osmosis.

This statement is true. Edema is a medical condition characterized by the accumulation of excess fluid in the body's tissues, leading to swelling. In some cases, edema can result from the retention of water in tissue cells due to osmosis, often as a result of imbalances in osmotic pressure between the intracellular and extracellular compartments.

Based on the evaluation, the correct statements with reference to osmotic pressure are 3. A, B, C, and D.