Which of the following enzyme is used during isolation of DNA from plant cell?

Cellulase

The correct answer is Option (4) → Cellulase

Cellulase: In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macro-molecules. Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the plant tissue with enzymes such as cellulase. Cellulase is an enzyme that breaks down cellulose, a complex carbohydrate found in the cell walls of plants.

Pectinase is the collection of enzymes that catalyze the pectin-containing substances produced by plants and microbes.

Chitinase: Chitinase is an enzyme that breaks down chitin, a structural polysaccharide found in the cell walls of fungi and some bacteria.

Lysozyme: Bacterial cell walls contain peptidoglycan, and lysozyme is an enzyme that degrades peptidoglycan, thus breaking down bacterial cell walls.