Which of the following enzyme is used during isolation of DNA from plant cell?
Answer & explanation
Correct answer: option 4
The correct answer is Option (4) → Cellulase
Option (4) Cellulase — Correct. To isolate DNA, the cell wall must first be broken. Plant cell walls are made of cellulose, so plant cells are treated with cellulase; lysozyme is used for bacteria and chitinase for fungi.
NCERT Extract:
Isolation of the Genetic Material (DNA) – “… Recall that nucleic acid is the genetic material of all organisms without exception. In majority of organisms this is deoxyribonucleic acid or DNA. In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macro-molecules. Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).”