What will happen if more than one recognition sites are present in vector?

Generation of several fragments will occur.

If more than one recognition site is present in the vector, the generation of several fragments will occur.

In molecular biology and genetic engineering, restriction enzymes (also known as restriction endonucleases) are used to cut DNA at specific recognition sites. These recognition sites are usually palindromic sequences, meaning they read the same in the 5' to 3' direction on both strands of the DNA.

When a DNA molecule is treated with a restriction enzyme, it will be cleaved at each recognition site, resulting in the formation of DNA fragments. If a vector (a type of DNA molecule used for cloning) contains more than one recognition site for a particular restriction enzyme, the enzyme will cut the vector at each of these sites, generating several DNA fragments of varying lengths.

This can be problematic in gene cloning experiments, as multiple fragments can complicate the process of inserting a desired gene of interest into the vector. Therefore, for successful gene cloning, it is important to use a vector that contains a single recognition site for the chosen restriction enzyme, which will result in the generation of only one DNA fragment that can be easily ligated with the gene of interest.