In pBR322, the recognition site for Bam HI is located in which component?

Tetracycline resistant site

To facilitate the process of gene cloning, it is essential for the vector to have a limited number of recognition sites for commonly used restriction enzymes. Ideally, the vector should have only one recognition site or very few such sites. Having multiple recognition sites within the vector can result in the generation of several DNA fragments when the restriction enzyme cuts the DNA.

This situation can complicate the process of gene cloning because it becomes challenging to identify the desired fragment containing the foreign DNA amidst multiple fragments. It may also lead to the insertion of foreign DNA into unintended locations within the vector.

For effective gene cloning, the alien DNA is ligated (joined) to the vector at a specific restriction site. For example, you can introduce foreign DNA into the vector pBR322 by linking it at the BamH I site within the tetracycline resistance gene of the vector.