Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Read the passage carefully and answer the given questions.

Recombinant DNA Technology

Asexual reproduction preserves the genetic information, while sexual reproduction permits variation. Traditional hybridization procedures used in plants and animals breeding very often lead to inclusion and multiplication of undesirable genes along with the desired genes. The techniques of genetic engineering, which include creation of recombinant DNA, use of gene cloning and gene transfer, overcome this limitation and allow us to isolate and introduce only one or a set of desirable genes without introducing genes into the target organism.

Which one of the following techniques is used to amplify a specific DNA sequence?

Options:

Polymerase Chain Reaction (PCR)

Gel Electrophoresis

Northern Blotting

DNA Sequencing

Correct Answer:

Polymerase Chain Reaction (PCR)

Explanation:

The correct answer is Option (1) → Polymerase Chain Reaction (PCR) 

Polymerase Chain Reaction (PCR) is a widely used molecular biology technique that enables the selective amplification of a specific segment of DNA. PCR has become an essential tool in various fields, including genetics, genomics, forensics, and medical diagnostics.

The PCR process involves three main steps:

  1. Denaturation

  2. Annealing

  3. Extension:

The polymerase chain reaction (PCR) is a laboratory technique used for the amplification of DNA. It allows the selective and targeted amplification of a specific DNA segment, creating multiple copies of the DNA region of interest. PCR is widely used in various molecular biology applications, including DNA sequencing, genetic analysis, and diagnostics.