What is the consequence of having multiple recognition sites within the vector?

Generation of several fragments

The correct answer is Option (1) –Generation of several fragments

Cloning sites in a vector are specific DNA sequences that have very few, preferably single, recognition sites for commonly used restriction enzymes. These cloning sites are important because they allow researchers to link the alien DNA (the DNA fragment to be cloned) into the vector using the appropriate restriction enzyme.

Having only one or very few recognition sites within the vector is crucial for successful gene cloning. If the vector contains multiple recognition sites for the same restriction enzyme, it could result in the generation of several DNA fragments during the cloning process. This complicates the process of selecting the desired cloned gene and increases the chance of undesirable recombination events.

By having a single or very few cloning sites, researchers can ensure that the alien DNA is inserted at the desired location in the vector, leading to the generation of the desired recombinant DNA. This makes the cloning process more straightforward and efficient, reducing the chances of errors and simplifying the selection of the desired cloned gene.