Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Molecular Basis of Inheritance

Question:

Read the passage given below and answer the following question.
The process of copying genetic information from one strand of the DNA into RNA is termed as transcription. Here also, the principle of complementarity governs the process of transcription, except the adenosine complements now forms base pair with uracil instead of thymine. A transcription unit in DNA is defined primarily by the three regions in the DNA. There is a convention in defining the two strands of the DNA in the structural gene of a transcription unit. Since the two strands have opposite polarity and the strand that has the polarity 3'→5' acts as a template, and is also referred to as template strand. The other strand which has the polarity (5'→3') and the sequence same as RNA (except thymine at the place of uracil), is displaced during transcription. Strangely, this strand (which does not code for anything) is referred to as coding strand. The promoter and terminator flank the structural gene in a transcription unit. It is a DNA sequence that provides binding site for RNA polymerase, and it is the presence of a promoter in a transcription unit that also defines the template and coding strands.

The promoter is said to be located towards which site of the structural gene?(Reference is made with respect to the polarity of coding strand) (Question 2)

Options:

Towards 5'-end (upstream)

Towards 3'-end (upstream)

Towards 5'-end(downstream)

Towards 3'-end (downstream)

Correct Answer:

Towards 5'-end (upstream)

Explanation:

The correct answer is 1. Towards 5'-end (upstream).

In the context of the polarity of the coding strand, the promoter is located towards the 5'-end (upstream) of the structural gene. The promoter region is where RNA polymerase binds to initiate transcription, and it is typically found upstream of the coding region (towards the 5' end) on the DNA strand.