Arrange the following steps of rDNA technology in correct sequence.
(A) Amplification of gene by PCR
(B) Insertion of rDNA into host cell using vector
(C) Isolation of the genetic material from the cell
(D) Cutting the DNA at specific location
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → (C), (D), (A), (B)
DNA is first isolated (C), then cut at specific locations (D); the gene of interest is amplified by PCR (A); and the recombinant DNA is inserted into the host cell (B).
NCERT Extract:
Processes of Recombinant DNA Technology – “… Recombinant DNA technology involves several steps in specific sequence such as isolation of DNA, fragmentation of DNA by restriction endonucleases, isolation of a desired DNA fragment, ligation of the DNA fragment into a vector, transferring the recombinant DNA into the host, culturing the host cells in a medium at large scale and extraction of the desired product.”