Which of the following statements are incorrect?
(A) Fragments of DNA can be separated by ELISA.
(B) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium.
(C) Recombinant DNA technology does not involve isolation of a desired DNA fragment.
(D) DNA ligases are used for stitching DNA fragments into a vector.
Choose the answer from the options given below:
Answer & explanation
Correct answer: option 1
The correct answer is Option (1) -(A) and (C) only
A) Fragments of DNA can be separated by ELISA
This statement is incorrect. ELISA (Enzyme-Linked Immunosorbent Assay) is a technique used for detecting and quantifying proteins, antibodies, and hormones, not for separating DNA fragments. DNA fragments are typically separated using gel electrophoresis.
(B) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium.
This statement is correct. Transformation is a well-known method used in molecular biology to introduce foreign DNA into a bacterial cell.
(C) Recombinant DNA technology does not involve isolation of a desired DNA fragment.
This statement is incorrect. Recombinant DNA technology specifically involves the isolation of a desired DNA fragment, followed by its insertion into a vector for further study or manipulation.
(D) DNA ligases are used for stitching DNA fragments into a vector.
This statement is correct. DNA ligases are enzymes that facilitate the joining of DNA strands together by catalyzing the formation of phosphodiester bonds, thus "stitching" DNA fragments into a vector.