Match List-I with List-II
| List-I | List-II |
|---|---|
| Enzyme | Function |
| (A) Nuclease | (I) Synthesizes DNA from RNA |
| (B) Ligase | (II) Have both directions exonuclease activity and synthesize new polynucleotide |
| (C) DNA Polymerase I | (III) Degrades nucleic acids by breaking phosphodiester bonds |
| (D) Reverse Transcriptase | (IV) Joins DNA molecules together by formation of phosphodiester bonds |
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → (A)-(III), (B)-(IV), (C)-(II), (D)-(I)
(A) Nuclease — this matches (III) Degrades nucleic acids by breaking phosphodiester bonds. Nucleases cut the bonds that link nucleotides. Exonucleases remove nucleotides from the ends; endonucleases cut inside the DNA.
(B) Ligase — this matches (IV) Joins DNA molecules together by formation of phosphodiester bonds. Ligase does the reverse of a nuclease: it seals two DNA pieces into one.
(C) DNA Polymerase I — this matches (II) Have both directions exonuclease activity and synthesize new polynucleotide. It adds nucleotides to build DNA, and it can also remove nucleotides, which lets it correct mistakes and fill gaps.
(D) Reverse Transcriptase — this matches (I) Synthesizes DNA from RNA. It copies RNA into DNA, the reverse of transcription; HIV uses it to make viral DNA from its RNA.