In which two steps of glycolysis ATP is utilised ?

 First in the conversion of glucose into glucose 6-phosphate .

Second in the conversion of fructose 6-phosphate to fructose 1, 6-bisphosphate.

The correct answer is Option (3) - 

 First in the conversion of glucose into glucose 6-phosphate .

Second in the conversion of fructose 6-phosphate to fructose 1, 6-bisphosphate.

The term glycolysis has originated from the Greek words, glycos for sugar, and lysis for splitting. The scheme of glycolysis was given by Gustav Embden, Otto Meyerhof, and J. Parnas, and is often referred to as the EMP pathway. In anaerobic organisms, it is the only process in respiration.

Glycolysis occurs in the cytoplasm of the cell and is present in all living organisms. In this process, glucose undergoes partial oxidation to form two molecules of pyruvic acid. In plants, this glucose is derived from sucrose, which is the end product of photosynthesis, or from storage carbohydrates. Sucrose is converted into glucose and fructose by the enzyme, invertase, and these two monosaccharides readily enter the glycolytic pathway.  

During glycolysis ATP is utilised at two steps: first in the conversion of glucose into glucose 6-phosphate and second in the conversion of fructose 6-phosphate to fructose 1, 6-bisphosphate .